Jamestown is the home of the Jamestown Chiefs, semi-professional football team in the Northeastern Football Alliance (NFA).The Jamestown Chiefs were founded by Shane Salter Jr. in August 2008. Initially, the Jamestown Chiefs were called the Jamestown Scorpions and the team colors were Navy Blue and Silver. To honor the heritage of Chautauqua County, the team name was changed to the Jamestown Warriors in September 2008. In November 2008, the team decided to let the community vote on the team name and colors. At the end of November 2008, the voting ended and the team became the Jamestown Chiefs, and the colors became Navy Blue and Orange.

This is the home city for the Jamestown Jammers baseball team of the New York - Penn League. The Jammers are the Single A Short Season affiliate of the Florida Marlins.

Jamestown was also home to the now-defunct Jamestown Vikings minor-league hockey team, whose home arena was the Jamestown Savings Bank Ice Arena.

Jamestown is home to the Jamestown Jets of the United Junior Hockey League.

Jamestown hosted its 14th Babe Ruth World Series in August 2008.